Cre mRNA (Cap1, m1Ψ)

Cre is a tyrosine recombinase enzyme derived from the P1 bacteriophage. The cre-loxP-mediated recombination system is an integral experimental tool for mammalian genetic and cell biology. This mRNA is capped with Cap1 structure with high capping efficiency. It has 100% substituted with N1-methyl-pseudoUridine for enhanced expression and reduced immunogenicity. The mRNA has a 100A tail in its sequence, mimics a mature mRNA.

价格	￥1000
产品编号	RP-A00005-0.1
规格	0.1 mg 0.2 mg 1 mg 大单询价
数量

产品属性质控参数应用指导图例

Description

Cre is a tyrosine recombinase enzyme derived from the P1 bacteriophage. The cre-loxP-mediated recombination system is an integral experimental tool for mammalian genetic and cell biology. This mRNA features: - A Cap 1 structure with high capping efficiency - 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response - GenScript’s patented UTR to enhance translation - A 100A poly(A) tail to mimic natural mature mRNA 星空体彩app官方.

产品属性

Form	Liquid
Concentration	1mg/mL
Full mRNA length	1407nt
Full mRNA Molecular Weight	459549
Storage buffer	1mM Sodium citrate, pH6.5
Storage condition	Store at -20°C for short term (<3 months), store at -80°C for long term.

质控参数

Appearance	Clear and free of foreign particles
RNA Length	Expected size band detected
RNA Content	Target ± 5%
Integrity	≥ 75%
OD260/OD280	1.70 ~ 2.30
Capping Efficiency	≥ 90%
Endotoxin	< 10 EU/mg
pH	Target ± 0.5

应用指导

Transfect 0.1 ug of mRNA using 0.5 uL of Lipofectamine™ MessengerMAX™ Transfection Reagent (or equivalent) for one well of HEK293-LoxP-GFP-RFP(Bsd) cells in a 96 well plate following manufacture’s instruction. Detect the RFP expression on cells using plate reader at the excitation of 560nm, and emission at 620nm.

图例

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden 星空全站体育app. .