"星空手机网页版" GenLNP-L01-EGFP mRNA (m1Ψ)

价格	￥2000
产品编号	RP-A00013-50
规格	0.05 mg 大单询价
数量

产品属性质控参数应用指导图例

Description

The LNP formulation loaded with eGFP mRNA (m1Ψ) is prepared using generic LNP formulation with main ionizable lipid of LP01. The payload EGFP mRNA (m1Ψ) has all the U bases 100% modified with N1-methyl-pseudouridine (m1Ψ). It expresses an enhanced green fluorescent protein, with the open reading frame sequence originally from the jellyfish, Aequorea Victoria. EGFP mRNA is a commonly used fluorescence reporter sequence in mRNA therapeutics and delivery system development projects 星空体彩app官方. The expressed protein has an excitation wavelength of 488nm, emission at 507nm. This formulation is a good control for testing if LP01 LNP formulation is a good potential formulation for your mRNA product under research in your in vitro and in vivo experiment model.

产品属性

Form	Liquid
Concentration	0.15mg/mL
Full mRNA length	980nt
Full mRNA Molecular Weight	319792
Storage buffer	Tris-HCl/Sucrose
Storage condition	Store at -80°C for long term. Avoid freeze thaw, only thaw once before use.

质控参数

Appearance	Clear and free of foreign particles
Encapsulation Efficiency	> 85%
Endotoxin	< 4 EU/ml
Particle Size	65-125 nm
pH	7.4 ± 0.5
Polydispersity Index	< 0.2
Zeta Potential	± 15.0 mV

应用指导

1. LNP can be added directly to cells incubated in complete media
2. Cells grown and transfected in complete media (DMEM +10% FBS) has higher expression than reduced serum medium
3. For suspension cell lines, we recommend using low fluorescent background media such as Flourobrite® with 10% FBS, 1X GlutaMax® and 1X sodium pyruvate
4. For adherent cell line, we recommend replace complete media with Opti-mem right before reading the cell eGFP expression
5. For higher expression, we recommend incubate the LNP with FBS at 1:2 volume ratio (LNP:FBS) for 30 minutes at 37C before transfecting

图例

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden. 星空手机网页版.